While blocking antibodies are generally effective at reducing non-specific binding, there are potential pitfalls to be aware of. Over-blocking can sometimes occur, where the blocking agent itself may interfere with the binding of the primary antibody to its target antigen. It is also important to use an appropriate blocking agent that is compatible with the antibodies and tissues being used. Finally, the effectiveness of blocking can vary depending on the specific conditions and reagents used, so optimization may be necessary.
