Fluorescent dyes are employed in various histological techniques:
1. Immunofluorescence: This technique uses antibodies conjugated with fluorescent dyes to detect specific proteins in tissue sections. 2. Fluorescent In Situ Hybridization (FISH): Utilizes fluorescent probes to bind specific DNA sequences, allowing for the localization of genes and chromosomal abnormalities. 3. Live Cell Imaging: Dyes can label living cells to observe dynamic processes in real-time.
