The preparation of epoxy resins involves mixing the resin with a curing agent and sometimes an accelerator. The mixture is then infiltrated into the tissue sample and allowed to polymerize. The following steps are typically involved:
Fixation of the tissue to preserve cellular structures. Dehydration of the tissue using a series of alcohols to remove water. Infiltration with the epoxy resin mixture, ensuring thorough penetration. Polymerization, where the resin hardens to form a solid block. Sectioning the hardened block using an ultramicrotome to obtain thin sections for analysis.
