How are Degenerative Changes Diagnosed in Histology?
Diagnosing degenerative changes in histology involves several techniques: - Light Microscopy: The most common method, where tissues are stained using dyes such as hematoxylin and eosin (H&E) to reveal cellular and structural details. - Special Stains: Techniques like Masson's trichrome, Periodic acid-Schiff (PAS), and Oil Red O can highlight specific tissue components or abnormalities. - Immunohistochemistry: Uses antibodies to detect specific proteins that may indicate degenerative changes. - Electron Microscopy: Provides detailed images of cellular ultrastructure, useful for identifying subcellular changes.
