How are BRAF mutations detected in histological samples?
BRAF mutations can be detected using various molecular techniques. Immunohistochemistry (IHC) is one method, where specific antibodies are used to detect the presence of the mutant B-Raf protein in tissue samples. Another method is polymerase chain reaction (PCR)-based assays that amplify and sequence the DNA to identify mutations. Next-generation sequencing (NGS) is also employed for a more comprehensive analysis.
