obscured details

Are there any limitations to these advanced techniques?

While advanced techniques can significantly improve the visibility of obscured details, they are not without limitations:
1. Cost: Advanced techniques like electron microscopy and confocal microscopy can be expensive and resource-intensive.
2. Complexity: These techniques often require specialized training and expertise.
3. Sample Preparation: Some advanced techniques necessitate complex sample preparation, which can introduce additional artifacts if not done correctly.
4. Availability: Not all laboratories have access to advanced microscopic equipment.

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