Several histological techniques are employed to assess ATP loss and its effects:
1. Histochemical Staining: Stains such as ATPase can identify areas of ATP activity or deficiency. 2. Electron Microscopy: This technique provides detailed images of mitochondrial structure and integrity. 3. Immunohistochemistry: Antibodies against mitochondrial proteins can help visualize mitochondrial health and ATP production levels. 4. Fluorescent Probes: Certain dyes can indicate cellular ATP levels and mitochondrial membrane potential.
