Cryo Electron Microscopy (cryo-EM) is an advanced imaging technique used to study the ultrastructure of biological specimens at cryogenic temperatures. Unlike traditional electron microscopy, cryo-EM preserves the native state of the sample by rapidly freezing it, thus avoiding the need for chemical fixatives or stains. This technique allows researchers to observe biological structures in their near-native environment, providing high-resolution images at the molecular and atomic levels.