Spectral overlap can lead to several issues in histological studies: - Reduced Signal Clarity: Overlapping emissions can cause signals to blend, making it challenging to distinguish separate structures or molecules. - Increased Background Noise: Unintended cross-talk between channels can elevate background noise, reducing the contrast of the image. - Quantification Errors: Overlapping signals can result in inaccurate quantification of the fluorophores or chromophores, leading to erroneous conclusions.
