Why is Multiphoton Microscopy Preferred for Live Tissue Imaging?
Multiphoton microscopy is preferred for live tissue imaging because it uses longer-wavelength, low-energy photons, which penetrate deeper into tissues with less damage. This minimizes phototoxicity and photobleaching, making it ideal for imaging living specimens over extended periods. It also allows for the excitation of fluorophores at deeper tissue levels, providing high-resolution images of thick specimens.
