How Does Super-Resolution Microscopy Break the Diffraction Limit?
Super-resolution microscopy techniques, such as STED, PALM, and STORM, break the diffraction limit of conventional light microscopy, which is approximately 200 nm. These methods use various strategies to achieve nanometer-scale resolution, allowing for the visualization of molecular details that were previously unresolvable. This has revolutionized our ability to study cellular structures and dynamics at the molecular level.