Several factors can contribute to inconsistent staining:
Fixation Issues: Inadequate or improper fixation can lead to poor preservation of tissue morphology, resulting in uneven staining. Reagent Quality: The quality and shelf-life of stains and other reagents can affect staining consistency. Tissue Processing: Inconsistent dehydration, clearing, or infiltration during tissue processing can lead to uneven staining. Section Thickness: Variability in section thickness can cause differences in stain uptake. Staining Protocol: Deviations from the standardized staining protocol can result in inconsistent outcomes.
