How Does Ficoll Work in Density Gradient Centrifugation?
Density gradient centrifugation using Ficoll involves the creation of a density gradient within a centrifuge tube. When a sample, such as blood, is added and subjected to centrifugation, the different cellular components move to positions where their densities match that of the Ficoll gradient. For example, mononuclear cells like lymphocytes and monocytes will concentrate at a specific layer, making them easier to collect.
