Fixation: Optimal temperatures are essential to preventing autolysis and preserving tissue architecture.
Embedding: Paraffin wax embedding requires precise temperatures for proper infiltration of tissues.
Sectioning: Cryostat sectioning and microtomy must be performed at controlled temperatures to achieve thin, uniform sections.
Staining: Many staining protocols require specific temperatures to ensure proper binding and visibility of stains.
Artifacts: Temperature fluctuations can cause artifacts that obscure diagnostic features.
Enzyme Activity Loss: Enzymes used in some stains or reactions may lose activity if not stored and used at the correct temperatures.
Sample Degradation: High temperatures can accelerate tissue degradation, while low temperatures can cause ice crystal formation in frozen tissues.
Thermometers: Used to manually check temperatures in different areas.
Data Loggers: Provide continuous temperature monitoring and recording.
Cryostats and
Incubators: These devices have built-in temperature control systems.
Regular Calibration: Ensure all temperature monitoring devices are regularly calibrated for accuracy.
Consistent Monitoring: Implement continuous monitoring systems to track temperature changes in real-time.
Routine Maintenance: Regularly maintain equipment to prevent breakdowns that could lead to temperature fluctuations.
Training Staff: Educate lab personnel on the importance of temperature control and proper handling of temperature-sensitive equipment.
Smart Sensors: These provide more accurate and real-time data on temperature conditions.
Wireless Monitoring: Enables remote tracking and alerts for temperature deviations.
Automated Systems: Integrated with laboratory information systems (LIS) to streamline data collection and analysis.
