Various types of substrates are used in histology, each serving a different purpose:
Chromogenic substrates: These substrates produce a colored product upon enzymatic reaction, allowing for visual localization of enzyme activity. Fluorogenic substrates: These substrates emit fluorescence when acted upon by specific enzymes, enabling visualization using fluorescence microscopy. Radioactive substrates: These substrates incorporate radioactive isotopes, facilitating detection through autoradiography. Substrate analogs: Modified substrates that mimic natural substrates, often used to study enzyme kinetics and inhibition.
