The administration of substrates can be performed through various methods, depending on the specific study and tissue type. Common techniques include:
In vitro incubation: Tissue sections are incubated with substrates in a controlled laboratory environment. In vivo administration: Substrates are introduced into living organisms through injection, ingestion, or other means. Microinjection: Direct injection of substrates into specific cells or tissues using fine needles or microcapillaries. Perfusion: Circulating substrates through the vascular systems of tissues or organs to ensure uniform distribution.