What types of microscopes are used in classical histology?
Several types of microscopes are employed in histology to observe tissue samples at different magnifications and resolutions:
Light Microscope: The most commonly used microscope in histology. It uses visible light to magnify tissue samples up to 1000 times, suitable for examining cell and tissue architecture. Electron Microscope: Provides much higher magnification and resolution compared to light microscopes. It is used to observe ultrastructures of cells, such as organelles. There are two main types: Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM). Fluorescence Microscope: Uses high-intensity light to excite fluorescent stains in tissues, allowing for the visualization of specific proteins, nucleic acids, or other molecules within cells.