Despite its advantages, 3D reconstruction in histology faces several challenges:
- Technical Complexity: The process requires sophisticated equipment and software, as well as expertise in both histological techniques and computational analysis. - Time-Consuming: The preparation, imaging, and reconstruction of samples can be time-intensive. - Data Management: Handling the large volumes of data generated during the process requires robust data storage and management solutions. - Image Artifacts: Issues such as sectioning artifacts, staining inconsistencies, and misalignment can affect the accuracy of the final 3D model.
