How are Specific Proteins Detected in Histological Samples?
Specific proteins can be detected in histological samples using various techniques, including:
- Immunohistochemistry (IHC): This technique uses antibodies specific to the protein of interest to visualize its localization and abundance in tissue sections. IHC is highly specific and sensitive, making it a powerful tool for diagnostic pathology. - Western Blot: While not a histological technique per se, Western blotting can be used to confirm the presence and quantify specific proteins extracted from tissues. - Fluorescence Microscopy: Fluorescently labeled antibodies or dyes are used to stain specific proteins, allowing their visualization under a fluorescence microscope. This technique can provide detailed information about protein distribution and co-localization with other cellular components.
