What Techniques are Used to Study TEWL Histologically?

Histologists use various staining and imaging techniques to assess the skin's barrier function:

1. Hematoxylin and Eosin (H&E) Staining: This common staining method can highlight the overall structure of the epidermis and the integrity of the stratum corneum.
2. Lipid Staining: Techniques like Oil Red O staining can specifically highlight the lipid content in the stratum corneum.
3. Electron Microscopy: Provides a detailed view of cell junctions, lipid layers, and other ultrastructural features affecting TEWL.
4. Immunohistochemistry: Can be used to detect proteins involved in cell cohesion and barrier function, such as filaggrin and loricrin.