What Techniques are Used to Study Protein Expression?
Several techniques are employed to study protein expression in histology:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections. The bound antibodies are then visualized using chromogenic or fluorescent labels. Western Blotting: Although not a histological technique per se, it is often used in conjunction with histology to quantify protein levels in tissue extracts. In Situ Hybridization (ISH): This method detects specific nucleotide sequences within tissue sections, providing information about gene expression that can be correlated with protein expression. Mass Spectrometry: Advanced mass spectrometry techniques can be used to identify and quantify proteins in tissue samples.
