What Techniques are Used to Study Nicastrin in Histology?
Several histological techniques are employed to study nicastrin, including:
1. Immunohistochemistry (IHC): This technique uses antibodies specific to nicastrin to visualize its distribution in tissues. 2. Western Blotting: Used to detect and quantify nicastrin protein levels in tissue extracts. 3. In situ Hybridization: This technique can be used to localize nicastrin mRNA within tissue sections. 4. Confocal Microscopy: Provides high-resolution images to study the subcellular localization of nicastrin.