Several techniques are employed to study differential gene expression in a histological context:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins in tissue sections, revealing patterns of gene expression. In situ hybridization (ISH): A method to visualize specific RNA transcripts within tissues, providing spatial information about gene expression. RNA sequencing (RNA-seq): A high-throughput approach to quantify and compare RNA levels across different conditions or tissues. Microarray analysis: This technique measures the expression levels of thousands of genes simultaneously using hybridization-based methods.