What Techniques are Used to Study Epigenetic Heterogeneity?

Several advanced techniques are employed to study epigenetic heterogeneity, including:

1. Single-cell RNA sequencing (scRNA-seq): This technique allows for the analysis of gene expression at the single-cell level, providing insights into the epigenetic landscape of individual cells within a tissue.
2. Chromatin immunoprecipitation followed by sequencing (ChIP-seq): This method is used to study protein-DNA interactions, such as histone modifications, providing a detailed view of chromatin states.
3. ATAC-seq: Assay for Transposase-Accessible Chromatin using sequencing is used to assess chromatin accessibility, which can reflect epigenetic modifications.
4. Bisulfite sequencing: This technique is used to detect DNA methylation patterns across the genome.