Several advanced techniques are employed to study enhancer boxes, including:
1. Chromatin Immunoprecipitation (ChIP): This technique is used to identify DNA sequences bound by specific transcription factors in vivo. 2. Reporter Assays: These assays involve cloning putative enhancer sequences upstream of a reporter gene to study their regulatory effects. 3. CRISPR-Cas9: This genome-editing tool can be used to delete or modify enhancer sequences to study their functional roles.
