Various techniques are employed to study actin binding in histology. Fluorescence microscopy, including confocal and super-resolution microscopy, allows visualization of actin filaments and ABPs in cells. Biochemical assays, such as co-sedimentation and fluorescence anisotropy, help quantify the binding affinities of ABPs to actin. Advanced methods like cryo-electron microscopy provide detailed structural insights into actin-ABP complexes.
