Histologists use various techniques to study chemical mediators in tissues:
Immunohistochemistry (IHC): This technique involves the use of antibodies to detect specific antigens (such as cytokines or growth factors) in tissue sections. In situ hybridization: This method detects specific nucleic acid sequences in tissue sections, useful for identifying the mRNA of chemical mediators. Enzyme-linked immunosorbent assay (ELISA): Though not a histological technique per se, ELISA is often used to quantify the levels of chemical mediators in tissue extracts.
