What Techniques are Used to Detect Cytogenetic Markers?
Several techniques are employed to detect cytogenetic markers:
Fluorescence in situ hybridization (FISH): This technique uses fluorescent probes that bind to specific DNA sequences, allowing visualization under a fluorescence microscope. Karyotyping: This involves staining chromosomes to observe their number and structure under a light microscope. Polymerase Chain Reaction (PCR): PCR amplifies specific DNA sequences, making it easier to study genetic material. Comparative Genomic Hybridization (CGH): This technique compares the DNA content of a test sample to a reference sample to detect genetic imbalances.
