Several techniques are employed in proteomics, including:
- Mass Spectrometry (MS): This is a pivotal technique used to identify and quantify proteins in complex samples. MS can analyze protein modifications, such as phosphorylation and glycosylation. - Two-Dimensional Gel Electrophoresis (2D-GE): This technique separates proteins based on their isoelectric point and molecular weight. - Liquid Chromatography (LC): Often coupled with MS, LC helps in separating proteins or peptides before analysis. - Western Blotting: Used for the detection and quantification of specific proteins within a sample.
