Several techniques are employed to integrate metabolomics with histology: - Mass Spectrometry Imaging (MSI): This technique provides spatial distribution of metabolites directly from tissue sections, allowing for the correlation of metabolic data with histological features. - Nuclear Magnetic Resonance (NMR) Spectroscopy: NMR can be used to analyze metabolites in tissue extracts, offering high reproducibility and quantitative accuracy. - Liquid Chromatography-Mass Spectrometry (LC-MS): LC-MS is a powerful technique for identifying and quantifying metabolites in tissue samples, providing detailed metabolic profiles.
