What Techniques Are Used in Histology to Study Molecular Mechanisms?
Several advanced techniques are employed to study molecular mechanisms within tissues. These include:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific antigens in tissue sections, providing insights into the distribution and localization of proteins. In Situ Hybridization (ISH): ISH allows for the detection of specific nucleic acid sequences within tissues, giving a spatial context to gene expression. Fluorescence Microscopy: This technique utilizes fluorescent dyes to label and visualize various molecular components within cells and tissues. Electron Microscopy: Provides high-resolution images of cellular and subcellular structures, revealing detailed molecular architecture.