In vivo studies in histology often involve several key steps: - Animal Models: Most in vivo studies are conducted using animal models such as mice, rats, and zebrafish. These models are chosen based on their relevance to human physiology and disease. - Imaging Techniques: Techniques such as live-cell imaging, multiphoton microscopy, and MRI are used to visualize tissues in real-time. - Histological Staining: After the in vivo experiment, tissues are often harvested and stained using techniques like Hematoxylin and Eosin (H&E), immunohistochemistry, or fluorescent dyes to reveal detailed tissue architecture and cellular composition.