What Techniques are Used in Histology for Biomarker Discovery?
Several advanced techniques are employed in histology to discover and validate new biomarkers. Some of these include:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific antigens in tissue sections, helping to identify and localize biomarkers. In Situ Hybridization (ISH): This method allows for the detection of specific nucleic acid sequences within tissue sections, providing insights into gene expression. Mass Spectrometry: Used in conjunction with histology, this technique can identify and quantify proteins and other molecules in tissue samples. Fluorescence Microscopy: This technique enables the visualization of fluorescently labeled molecules in tissues, aiding in the identification of biomarkers.
