What Techniques are Used in Histological Investigation?
Various techniques are employed in histological investigations, including:
Tissue Fixation: Preserves the tissue's structure and prevents decay. Tissue Embedding: Involves embedding tissues in a solid medium, usually paraffin wax, to facilitate sectioning. Microtomy: The process of cutting thin tissue sections for microscopic examination. Staining: Applying dyes to enhance tissue contrast and highlight different structures. Common stains include Hematoxylin and Eosin (H&E), Masson's Trichrome, and Periodic Acid-Schiff (PAS). Immunohistochemistry (IHC): Uses antibodies to detect specific proteins within tissues, providing insights into protein expression and localization.
