What Techniques are Used in Gene Expression Profiling?
Several techniques are employed for gene expression profiling, including:
1. Microarray Analysis: Uses DNA microarrays to measure the expression levels of many genes simultaneously. 2. RNA-Seq: A next-generation sequencing (NGS) technique that provides a comprehensive view of the transcriptome. 3. qPCR: Quantitative PCR is used for validating gene expression levels identified by other methods. 4. In Situ Hybridization (ISH): Detects specific nucleic acid sequences within the histological sections.
