Several techniques are employed for 3D imaging in histology, including:
Confocal Microscopy: Uses laser scanning and optical sectioning to create detailed 3D images. Multiphoton Microscopy: Allows imaging of deeper tissue layers with less photodamage. Light Sheet Fluorescence Microscopy: Provides high-speed, high-resolution imaging of large samples. Micro-CT: Uses X-ray tomography for high-resolution 3D imaging of tissues.
