What Techniques are Employed in Histological Analysis?
Histological analysis employs several key techniques to study tissues:
Fixation: This process preserves tissue morphology by stabilizing cellular structures. Common fixatives include formaldehyde and glutaraldehyde. Embedding: Tissues are embedded in paraffin wax or resin to provide support for thin sectioning. Sectioning: Thin slices of tissue are cut using a microtome or ultramicrotome for examination under a microscope. Staining: Various stains are applied to enhance the contrast between different tissue components. Common stains include H&E, PAS, and Masson's trichrome.
