What Techniques Are Commonly Used in Histological Analysis of Drug Effects?
Several histological techniques are employed to assess drug effects, including:
Hematoxylin and Eosin (H&E) staining: This common staining method provides a general overview of tissue architecture and cellular structures. Immunohistochemistry (IHC): This technique uses antibodies to detect specific proteins within tissues, helping to identify cellular targets of drugs. In Situ Hybridization (ISH): Used to detect specific nucleic acid sequences within tissues, useful for studying the expression of genes affected by drugs. Electron microscopy: Provides high-resolution images of cellular organelles, aiding in the detailed analysis of drug-induced changes at the ultrastructural level.
