Technical variability often arises from the methods and protocols used in preparing tissue samples. Key steps that introduce variability include:
Fixation: Inadequate or prolonged fixation can result in poor preservation of tissue architecture and antigenicity. Sectioning: Variations in the thickness of tissue sections can lead to inconsistencies in staining and morphology. Staining: Differences in staining protocols, reagent quality, and staining duration can lead to variable results.
Standardizing these processes is crucial to minimize technical variability and ensure reproducibility in histological analysis.
