Staining is essential for highlighting specific structures within a tissue sample, but it can introduce bias if not performed correctly. Different tissues and cell types may require specific stains, such as Hematoxylin and Eosin (H&E), which are standard for general tissue visualization. However, the intensity and uniformity of staining can vary, affecting the ability to distinguish between different tissue components. Variability in staining can also arise from differences in reagent batches and staining protocols.
