Several sources can contribute to technical bias in histology:
1. Sample Preparation: The initial steps, such as fixation, embedding, and sectioning, can introduce bias. Poor fixation can lead to tissue shrinkage or distortion, while improper embedding or sectioning can result in uneven slices that do not accurately represent the tissue.
2. Staining Techniques: The choice of staining method and the quality of reagents can impact the visibility and contrast of tissue structures. Over-staining or under-staining can obscure important details, leading to misinterpretation.
3. Microscope Calibration: Incorrect calibration of microscopes, including lighting and magnification settings, can cause discrepancies in the observation and documentation of tissue features.
4. Human Interpretation: Observer bias is a significant factor, as different individuals may perceive and interpret histological images differently based on their experience and expectations.
