What Methods are Used to Study Cell Adhesion in Histology?
Several techniques are employed to study cell adhesion in histology:
Immunohistochemistry (IHC) - Uses antibodies to detect specific CAMs in tissue samples, providing spatial and quantitative information. Fluorescence microscopy - Allows visualization of CAMs and their interactions in live or fixed tissues using fluorescently labeled molecules. Western blotting - Analyzes the expression levels of CAMs in tissue extracts. Flow cytometry - Quantifies CAMs on the surface of cells isolated from tissues. Atomic force microscopy (AFM) - Measures the mechanical forces involved in cell adhesion.