Preparation of biological samples involves several steps to ensure they are suitable for microscopic examination. These include:
Fixation: Preserving the tissue using chemicals like formalin to prevent decay. Dehydration: Removing water from the tissue using alcohols. Embedding: Enclosing the tissue in a medium like paraffin wax for support during sectioning. Sectioning: Cutting thin slices of the tissue using a microtome. Staining: Applying dyes to enhance contrast and highlight specific structures.