Fixation: The tissue is first fixed using chemicals like formaldehyde to preserve its structure. Graded Ethanol Series: The tissue is then passed through a series of ethanol solutions, starting from a lower concentration (50%) and gradually moving to absolute ethanol (100%). Clearing: After dehydration, the tissue is treated with a clearing agent like xylene to remove the alcohol and make the tissue transparent. Embedding: Finally, the tissue is embedded in paraffin wax or another suitable medium, ready for sectioning.
