verhoeff van gieson

What is the staining protocol?

The protocol for VVG staining involves several steps:
Deparaffinize and rehydrate tissue sections.
Stain with Verhoeff's solution.
Differentiation using a solution of ferric chloride until elastic fibers are distinct.
Counterstain with Van Gieson's solution.
Dehydrate, clear, and mount the sections.

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