wavelength

What is the Role of Wavelength in Microscopy?

Different types of microscopy techniques utilize various wavelengths to achieve optimal visualization:
Brightfield Microscopy: Uses visible light (400-700 nm) for general tissue observation.
Fluorescence Microscopy: Employs specific wavelengths to excite fluorophores, causing them to emit light at a different wavelength, thereby illuminating specific components of the tissue.
Confocal Microscopy: Utilizes laser light of specific wavelengths to create high-resolution, three-dimensional images of tissues.

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