How Do Wavelengths Impact Fluorescence Microscopy?
In fluorescence microscopy, specific wavelengths are used to excite fluorophores in the sample. Each fluorophore has an excitation wavelength and an emission wavelength. The excitation wavelength is the specific wavelength of light absorbed by the fluorophore, while the emission wavelength is the light emitted. This property allows for the visualization of multiple targets within a single sample using different fluorophores.
