The procedure for formalin fixation typically involves the following steps:
Collecting the tissue sample promptly to prevent autolysis. Immersing the tissue in 10% neutral buffered formalin for a period sufficient to fix the tissue completely, usually 24 to 48 hours. Rinsing the tissue in water or a buffer solution to remove excess formalin. Processing the tissue through a series of alcohol and xylene baths to dehydrate and clear the sample before paraffin embedding.
