Multiphoton excitation is a fluorescence imaging technique that uses the simultaneous absorption of two or more photons to excite a fluorophore. Unlike traditional single-photon excitation, which uses a single high-energy photon, multiphoton excitation uses lower-energy photons in the near-infrared range. This technique is especially valuable in histology for its ability to image deeper into biological tissues with reduced photodamage and photobleaching.
