What Are the Advantages of Multiphoton Excitation in Histology?
Multiphoton excitation offers several advantages in histology:
Deeper tissue penetration: Near-infrared light used in multiphoton excitation penetrates deeper into tissues than visible light, allowing for imaging of thicker specimens. Reduced photodamage: Lower energy photons cause less damage to biological samples, preserving the integrity of delicate tissues. Reduced photobleaching: Because excitation is confined to the focal point, regions outside the focal plane experience less light exposure, minimizing photobleaching of fluorophores. Intrinsic optical sectioning: The nonlinear nature of multiphoton excitation provides inherent optical sectioning capability, eliminating the need for physical sectioning of tissues and improving three-dimensional imaging.
